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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: human
TISSUE/ORGAN: lung, embryonic
ECACC catalog code: 85020202
Morphology: Fibroblast
Description: Human Caucasian fetal lung
Viruses: See Properties
Depositor: Dr P Jacobs, NIBSC, London, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: rhinoviruses, production of viral vaccines
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 21;
Karyotype: 2n = 46, diploid
Established from normal lung tissue of a 15 week old female Caucasian foetus. Like MRC-5 the line is susceptible to a wide range of viruses. At least 40 population doublings expected.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 212
Bibliographic references:
- J Biol Stand 1979;7:113 - PMID: 113411
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it