MPK

Version 4.0.200811

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MPK (minipig, kidney)

SPECIES: minipig
TISSUE/ORGAN: kidney

ECACC catalog code: 87032604
Morphology: Fibroblast
Susceptibility to viruses: arbovirus * herpes simplex * hog cholera virus * leptospires * pig parvovirus * swine pestis virus * vesic. stomatitis (Indiana) * Visna
Description: Minipig kidney
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: arboviruses

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 20% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 30; Karyotype: 2n = 38, diploid
Established from the kidney of a 118 day old foetal minipig. The cells are susceptible to some arboviruses and leptospiras and highly refractory to Chlamydia spp. and meningopneumonitis.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 166, IZSBS: BS CL67

Bibliographic references:

Microbiologica 1987;10:301 - PMID: 3626887
Microbiologica 1988;11:263 - PMID: 3173126
Microbiologica 1988;11:371 - PMID: 3063925