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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: ATL97003
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: mouse, CD-1 transgenic (male, 5 months old)
TISSUE/ORGAN: mammary gland
TUMOR: adenocarcinoma
TRANSFORMED BY: rat activated neu oncogene Back To Oncogene Agents' WebPage
DEPOSITOR: Dr M.G. Sacco, Advanced Biomedical Technologies Institute, CNR, Milan, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: estrogen and progestinic receptor; Neu over expression; sensitive to anti-estrogens and apoptosis inducing.
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
William's medium + 15% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split cultures 1:3-1:6 using trypsin/EDTA do not allow to reach confluence; seed at 3-5x104 cells/cm2; 37C, 5% CO2. Passage: 23, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with MD, NP)
Bibliographic references:
- Breast Cancer Res Treat 1998;47:171-180 - PMID: 9497105
- Gene Ther 1998;5:388-393 - PMID: 9614559
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it