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SPECIES: human, Black
ECACC catalog code: 92020422
Description: Human Negroid breast medulla carcinoma
Viruses: See Properties
Depositor: Not registered
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
L-15 + 2mM Glutamine + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6, i.e. seeding at 3-6x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 37C. CO2 is not required.
Karyotype: Range 52-69
Isolated from pleural effusions of a breast cancer patient. Desmosomes, microvilli and tonofilaments observed at boundaries between cells.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: HTB 24
- In Vitro 1974;9:239 - PMID: 4471183
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293