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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL95017
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old)
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
Derived from: pleural effusion
Original line: MCF7
Transfected by: ER-pBact Back To Transfection WebPage
DEPOSITOR: Dr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa Italy.
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: estrogen receptor transgene
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2., mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with G6PD, NP)
See also
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it