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SPECIES: rat, buffalo
ECACC catalog code: 90021504
Description: Rat Buffalo hepatoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Alpha-foetoprotein Applications: Tumourigenicity and oncogeny studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Swimm's S-77 + 4mM Glutamine + 20% Horse Serum (HS) + 5% Fetal Bovine Serum (FBS) or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Fetal Bovine Serum (FBS). Split semi-confluent cultures 1:2 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C. NB: Cells are shed from the monolayer before confluence is achieved.
Derived from the Morris hepatoma 7777 (obtained from a male Buffalo strain rat). The original tumour was induced with N-2 fluorenylphthalamic acid. Particularly interesting for oncogeny studies since the cells produce alpha-fetoprotein (AFP).
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1601
- J Biol Chem 1982;257:5827 - PMID: 6175632
- Onco-Developmental Gene Expression (Fishman and Sell ed, Academic Press N Y) 1976;259-270
- Recent Results Cancer Res 1974;44:103
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293