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SPECIES: mouse, C57BL
TUMOR: carcinoma, Lewis lung
ECACC catalog code: 90020104
Description: Mouse C57BL Lewis lung carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity, Metastasis and chemotherapeutic agent studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using mechanical technique (aspiration); 5% CO2; 37C.
Derived from the lung of a C57BL mouse implanted with a primary Lewis Lung carcinoma. The cells can be used as a model for metastasis and for studying the effects of chemotherapeutic agents.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1642
- Cancer Lett 1980;11:63 - PMID: 7226139
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293