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SPECIES: mouse, BALB/c
ECACC catalog code: 89092101
TRANSFORMED BY: Kirsten MSV
Description: Mouse A31 embryo Kirsten sarcoma, transformed
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Contact inhibition, tumourigenicity and virus studies: MuLV, MSV
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Calf Serum (CS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.1% trypsin for 3-5 minutes; 5% CO2; 37C.
Karyotype: 2n = 40
Cell morphology is different from the parent clone (A31) and there is a loss of sensitivity to contact inhibition of cell division. The cells are highly tumorigenic. Superinfection with MuLV results in rescue of MSV.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 163.3
- J Gen Virol 1971;13:245 - PMID: 4333712
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293