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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: mouse, LAF1
TUMOR: pituitary tumor
ECACC catalog code: 87021902
Morphology: Aggregates
Description: Mouse LAF1 pituitary gland tumour
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Adrenocorticotropic hormone (ACTH) Applications: Hormone studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Fetal Bovine Serum (FBS). Allow clusters of cells to settle, remove supernatant leaving 5-10ml of growth medium in a 25cm² tissue culture flask, to cover aggregate. Gently remove clusters and divide equally in to 2 25cm² flasks and add fresh medium to each; 5% CO2; 37C Cells do not form monolayers, but tend to grow in small floating clusters and are subcultured as intact aggregates.
Passages: 45;
Karyotype: Diploid
Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 89
Bibliographic references:
- Proc Natl Acad Sci U S A 1962;48:1184-1190
- Proc Soc Exp Biol Med 1953;84:253-254
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it