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ECACC catalog code: 93122325
Description: Porcine thyroid epithelial
Viruses: See Properties
Depositor: Dr G Fayet/Dr S Hovsepian, Laboratoire de Biochimie Medicale, Faculte de Medecine, Marseille, F-13385, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone Products: Thyroglobulin, plasminogen activator (u-PA) Applications: Mechanism of thyroid follicle reconstruction
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Click:RPMI (1:1) + 10µg/ml Insulin + 10mM Hydrocortisone + 5µg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 2mM Glutamine + 0.1% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 i.e. seeding at 5x100,000 cells/cm2 using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO2; 37C.
The cell line was spontaneously derived from the porcine thyroid cell line PORTHOS (ECACC No. 93122323) after withdrawal of thyroid stimulating hormone (TSH). The cells form follicle-like structures, have a high growth rate, a high glucose consumption and are TSH independent for growth. The expression of plasminogen activators has been reported and found to be higher in ATHOS cells compared to PORTHOS cells.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Cell Mol Biol 1993;39:427
- Horm Metab Res 1993;25:238 - PMID: 8514246
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293