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ICLC catalog code: HTL97012
Continuous culture, grown as semiadherent
small cells morphology
SPECIES: human (male)
TUMOR: carcinoma, small cell
Derived from: metastatic lymph node
DEPOSITOR: Dr S. Ferrini Immunopharmacology Dept, IST at ABC, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:2-1:3 using trypsin/EDTA; seed at 2-4x104 cells/cm2; The cells detach very easily but are difficult to disaggregate; 37C, 5% CO2. Passage: 51, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with MD, NP.)
- Br J Cancer 1996;74:788-795 - PMID: 8795583
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293