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TISSUE/ORGAN: skin, fibroblast
ECACC catalog code: 90020509
Transfected by: Transfection Back To Transfection WebPage
Description: Human skin fibroblast, transformed
Viruses: See Properties
Depositor: Dr C Arlett, MRC Cell Mutation Unit, University of Sussex, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: DNA repair and cell growth studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from a Normal fibroblast (1BR3 ECACC No. 90011801) transformed with the plasmids pSV3gpt and pSV3neo. The cells were selected for pSV2neo only.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Carcinogenesis 1990;11:185 - PMID: 2153058
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293