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SPECIES: human, Caucasian
TISSUE/ORGAN: intestine, embryonic
ECACC catalog code: 85051004
Susceptibility to viruses: adenovirus 3 * Coxsackie B * mumps * Newcastle disease virus * poliovirus 1 * poliovirus 2 * poliovirus 3 * vesic. stomatitis (Indiana)
Description: Human Caucasian embryonic intestine, HeLa characteristics
Viruses: See Properties
Depositor: Dr D Newell, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 46, HeLa markers
Derived from the jejunum and ileum of a 2 month old Caucasian embryo. The cells have a similar virus susceptibility as HeLa but show less sensitivity. They carry HeLa marker chromosomes This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 6
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293