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TUMOR: pancreatic tumor
ECACC catalog code: 93100618
Morphology: Pancreas cells
Description: Rat exocrine pancreatic tumour
Viruses: See Properties
Depositor: Dr S Watson, Department of Surgery, Queens Medical Center, Nottingham, UK
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin Products: Amylase and exocrine enzymes Applications: Enzyme and receptor studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Cells grow in hollow spheroid colonies that can attach loosely. Split cultures 1:2 every 48 hours into fresh flasks, maintain cultures between 1-9x100,000 cells/ml. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be expected during culture.
Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1492
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293