AR42J

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

AR42J (rat, pancreas, pancreatic tumor)

SPECIES: rat
TISSUE/ORGAN: pancreas
TUMOR: pancreatic tumor

ECACC catalog code: 93100618
Morphology: Pancreas cells
Description: Rat exocrine pancreatic tumour
Viruses: See Properties
Depositor: Dr S Watson, Department of Surgery, Queens Medical Center, Nottingham, UK
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin Products: Amylase and exocrine enzymes Applications: Enzyme and receptor studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Cells grow in hollow spheroid colonies that can attach loosely. Split cultures 1:2 every 48 hours into fresh flasks, maintain cultures between 1-9x100,000 cells/ml. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be expected during culture.
Passages: 8-13; Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1492

Bibliographic references:

In Vitro 1980;16:212