AK-D

Version 4.0.200811

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AK-D (cat, lung, embryonic)

SPECIES: cat
TISSUE/ORGAN: lung, embryonic

ECACC catalog code: 89071903
Morphology: Epithelial
Susceptibility to viruses: feline herpes * feline panleukopenia * feline picornavirus
Description: Fetal cat lung normal
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: panleucopenia, feline herpes and feline picornaviruses

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin and 0.03% EDTA; 5% CO2; 37C.
Passages: 40; Karyotype: Diploid
A clonal derivative of mass cultures of foetal feline lung after 14 passages in vitro. Susceptible to panleukopenia, feline herpes and feline picornaviruses.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 150, IZSBS: CL 80

Bibliographic references:

Lab Invest 1976;34:494