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SPECIES: mouse, C3H/An
TISSUE/ORGAN: areolar and adipose
ECACC catalog code: 85011426
Description: Mouse C3H/An areola and adipose tissue
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Metabolic studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) or DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derivative of L929 selected in azaguanine (3µg/ml). Partially resistant to azaguanine. A9 may be heterozygous for the ability to synthesize active inosinic acid phosphorylase.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 6319
- Am J Hum Genet 1974;26:273
- Nature 1964;202:1142
- Proc Natl Acad Sci U S A 1963;50:568
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293