A9

Version 4.0.200811

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A9 (mouse, C3H/An, areolar and adipose)

SPECIES: mouse, C3H/An
TISSUE/ORGAN: areolar and adipose

ECACC catalog code: 85011426
Morphology: Fibroblast
Description: Mouse C3H/An areola and adipose tissue
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Metabolic studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) or DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Aneuploid
Derivative of L929 selected in azaguanine (3µg/ml). Partially resistant to azaguanine. A9 may be heterozygous for the ability to synthesize active inosinic acid phosphorylase.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 6319

Bibliographic references:

Am J Hum Genet 1974;26:273
Nature 1964;202:1142
Proc Natl Acad Sci U S A 1963;50:568