A8/DI

Version 4.0.200811

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A8/DI (mouse, AKR, thymoma)

SPECIES: mouse, AKR
TUMOR: thymoma

ECACC catalog code: 94031011
Morphology: Lymphocyte
TRANSFORMED BY: Radiant Agent Back To Radiant Agents' WebPage
Description: Mouse, AKR, thymoma
Viruses: See Properties
Depositor: Dr D Iannelli, University of Naples, School of Agriculture, ITALY (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Study of the assembly and transport of Ly-2/3 antigen in the cell surface or to define the regions of Ly-2/3 interacting with the corresponding antibodies and with MHC class I molecules.

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 10% Horse Serum (HS). Maintain cultures between 3-9x100,000 cells/ml; 5-10% CO2; 37C.
The parent cell line AKR/1a murine T cell line was exposed to a dose of 500 rads from an X-ray source. The mutant Lyt2- cells were selected out by magnetic bead separation.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ICLC: ATL98012

Bibliographic references:

Immunogenetics 1994;40:151
J Immunol Methods 1992;154:211 - PMID: 1401954