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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: human, Caucasian
TISSUE/ORGAN: lung
TUMOR: carcinoma
DSMZ catalog code: ACC 107
Morphology: epithelial cells, growing adherently as monolayer
Susceptibility to viruses: adenovirus
Description: human lung carcinoma established from an explanted lung tumor which was removed from a 58-year-old Caucasian man in 1972; cells were described to induce tumors in athmyic mice and to synthesize lecithin confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
General Restrictions
Available in the following LABORATORY:
-
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS split confluent cultures 1:5 to 1:10 every 4-7 days using trypsin/EDTA; cells grow easily; seed out at ca. 1-2 x 106 cells/80 cm 2 + 10 ml medium at 37C with 5% CO2 about 20 x 106 cells/175 cm 2;
doubling time of ca. 30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human hypotriploid karyotype with 8% polyploidy; 65(59-66)<3n>XXY, -1, -3, -6, +12, -13, -15, -18, -19, -21, -22, +4m, der(6)t(1;6)(q11;q27), del(11)(q22/23); presence of large distinctive der(6) marker confirms identity of this c ll lin) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of MDH, NP) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CCL 185
Bibliographic references:
- Biochem J 1993;294(1):49-55 - PMID: 8395818
- Biochem Soc Trans 1992;20(2):136S
- Brain Res 1992;583(1-2):100-104 - PMID: 1324089
- Carbohydr Res 1992;234:237-346 - PMID: 1334801
- Eur J Pharmacol 1990;184(2-3):233-238 - PMID: 1964127
- Eur J Pharmacol 1991;208(3):265-268 - PMID: 1685127
- FASEB J 1993;7(14):1376-1380 - PMID: 8224610
- FEBS Lett 1990;260(1):91-94 - PMID: 2153579
- Int J Cancer 1976;17:62-70 - PMID: 175022
- J Biol Chem 1990;265(36):22282-22287 - PMID: 2176212
- J Biol Chem 1991;266(12):7876-7882 - PMID: 1902229
- J Biol Chem 1991;266(13):8431-8438 - PMID: 2022658
- J Biol Chem 1991;266(30):20519-20524 - PMID: 1657928
- J Biol Chem 1993;268(26):19411-19415 - PMID: 8396137
- J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
- J Neurochem 1991;57(3):963-969 - PMID: 1650398
- J Neurochem 1993;61(2):12-17 - PMID: 8515256
- J Pharmacol Exp Ther 1990;253(1):185-191 - PMID: 2329505
- J Pharmacol Exp Ther 1993;264(3):1121-1127
- Mol Cell Biol 1992;12(10):4742-4750 - PMID: 1328861
- Mol Pharmacol 1990;37(2):296-303 - PMID: 1689454
- Mol Pharmacol 1991;39(3):399-406 - PMID: 1706472
- Mol Pharmacol 1993;44(2):364-370 - PMID: 8394993
- Naunyn Schmiedebergs Arch Pharmacol 1992;346(1):51-56 - PMID: 1407005
- Nippon Yakurigaku Zasshi 1992;99(1):1-11 - PMID: 1313776
- Prostaglandins 1990;40(4):405-416 - PMID: 91126216
- Symp Soc Exp Biol 1990;44:147-156 - PMID: 2130511
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it