A549

Version 4.0.200811

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A549 (human, Caucasian, lung, carcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: lung
TUMOR: carcinoma

ECACC catalog code: 86012804
Morphology: Epithelial
Susceptibility to viruses: adenovirus
Description: Human Caucasian lung carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Lecithin, High amounts of de-saturated fatty acids. Applications: Enzymatic studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12K or DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 85; Karyotype: Hypotriploid
Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.
Research council deposit: No; Price Code: A;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 185, ICLC: HTL03001, IZSBS: TCL 101

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