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ECACC catalog code: 93112519
Description: Human ovarian carcinoma
Viruses: See Properties
Depositor: Dr T H Ward, Cell Culture Unit, Patterson Laboratories, Christie Hospital, Manchester, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Toxicity testing, cancer genetic studies.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 3-6x10,000cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
The A2780 human ovarian cancer cell line was established from tumour tissue from an untreated patient. Cells grow as monolayer and in suspension in spinner cultures. A2780 is the parent line to the cisplatin resistant cell line A2780 cis (ECACC No. 93112517) and the adriamycin resistant cell line A2780 ADR (ECACC No. 93112520).
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ICLC: HTL98008
- Cancer Res 1987;47:414 - PMID: 3539322
- Semin Oncol 1984;11:285 - PMID: 6385258
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293