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SPECIES: hamster, Chinese
ECACC catalog code: 89030316
Description: Hamster Chinese ovary
Viruses: See Properties
Depositor: Dr Pohjanpelto, Department of Virology, University of Helsinki, FINLAND (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Ornithine decarboxylase Applications: Enzymatic studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + Ham's F12 (without Putrescine) (1:1) + 0.1% BSA. Split confluent cultures 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using cold PBS and 0.25% cold trypsin. Place flasks at 4C for 5-10 min. or until cells detach; 5% CO2; 37C. Pre-soak flasks in porcine collagen (Sigma G-2625) overnight at room temperature or 2hr at 37C. Serum is only used to initially resuscitate from frozen and should be added prior to cryopreservation.
Overproduces ornithine decarboxylase in response to polyamine deprivation.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- J Biol Chem 1985;260:8532 - PMID: 3924915
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293