|
Version 4.0.200811 |
|
This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: rat, BDIX
TISSUE/ORGAN: brain
TUMOR: glioma
ECACC catalog code: 93040117
Morphology: Epitheloid
Description: Rat glioma
Viruses: See Properties
Depositor: Dr J Roscoe, Department of Histopathology, University College and Middlesex School of Medicine, London, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Experimental tumour models, carcinogenesis studies
Available in the following LABORATORY:
-
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:50 to 1:100 seeding at 5x10,000 to 1x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 52;
Karyotype: Modal No 42
The glioma cell line A10-85 is a subclone of A10 cells which originally emerged from the parent line IA2 by reducing the serum concentration from 15% to 10%. This parent line IA2 was derived from a rat brain glioma induced transplacentally by ethylnitroso urea (inbred BDIxrats). A10-85 cells form processes in response to cAMP. GAPDH can be induced by hydrocortisone succinate and good growth was obtained in semi-solid medium. Injection of cells into syngeneric animals resulted in the formation of malignant astrocytic tumours after short latent periods.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Bibliographic references:
- Br J Exp Pathol 1979;60:209 - PMID: 221002
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it