HTC

Version 4.0.200811

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HTC (rat, buffalo, ascitic fluid, hepatoma)

Monoclonal continuous culture, grown as suspension
epithelial-like morphology
SPECIES: rat, buffalo (male) Karyology: extra chromosomes 1 and 13
TISSUE/ORGAN: ascitic fluid
TUMOR: hepatoma
Derived from: Morris hepatoma 7288C
tumorigenic in syngeneic animals
Cell line available under some conditions.
PROPERTIES: biochemical markers; enzymology; toxicity screening; tumorigenicity; cytotoxicity; xenobiotic metabolism; dexamethasone p receptor

Available in the following LABORATORY:

Istituto Patologia Generale, Universita' degli Studi (GEUCC, Genova)
MEM + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-30, mycoplasma negative, HOECHST
Growth also as monolayer. Derived from hepatoma induced by N,N
Validated by: beuraldehyde dehydrogenase (high levels) * chromosome analysis (large metacentric chromosome 1)

Availability in cell line catalogues: ECACC: 93120108, ICLC: ATL95006

Bibliographic references:

Adv Cancer Res 1965;9:227 - PMID: 4285261
ATLA 1988;16:32
Proc Natl Acad Sci U S A 1966;56:269 - PMID: 4381784