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SPECIES: human, Caucasian
TUMOR: adenocarcinoma, grade II
ECACC catalog code: 91072201
Susceptibility to viruses: vesicular stomatitis
Description: Human Caucasian colon adenocarcinoma grade II
Viruses: See Properties
Depositor: Not registered
No restrictions. Patent: None Specified By Depositor
Properties: Products: Secretory component of Immunoglobulin A (IgA), Carcinoembryonic antigen (CEA) Applications: Tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
McCoy's 5a + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Karyotype: 2n = 46, hypertriploid
Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: HTB 38
- Cancer Res 1988;48:6193-6200 - PMID: 3167866
- Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293