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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL95007
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (male, 78 years old) Karyology: diploid to hyperdiploid
TISSUE/ORGAN: kidney
TUMOR: adenocarcinoma
non tumorigenic
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: chemotherapeutic agents; cytotoxicity; tumorigenicity
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-2x105 cells/cm2. 37C, 5% CO2. Passage: 53, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with MD, MPI)
Availability in cell line catalogues: IZSBS: TCL 117
Bibliographic references:
- J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
- Oncogene 1994;9(3):939-942 - PMID: 8108138
- Proc Natl Acad Sci USA 1987;84(22):7972-7976 - PMID: 2825172
See also
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it