This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: human, Caucasian
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, promyelocytic
ECACC catalog code: 88120803
Description: Human Caucasian promyelocytic leukaemia
Viruses: See Properties
Depositor: Dr G Brown/Dr C M Bunce, Immunology Department The Medical School, University of Birmingham, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Differentiation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO). Maintain cultures between 3-9x100,000 cells/ml. Do not allow the culture medium to become acidic or the cells will differentiate; 5% CO2; 37C.
HL60 induced to mature into neutrophils with 1.75% DMSO after treating with SV40. Fails to express SV40 specific T Ag suggesting that HL60 M2 represents expansion of sub-clones of HL60 with inherent restricted capacity for neutrophil differentiation.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Exp Hematol 1983;11:828 - PMID: 6641827
- Leuk Res 1982;6:491-498 - PMID: 6958930
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293