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SPECIES: human, Caucasian
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, promyelocytic
ECACC catalog code: 88120802
Morphology: Promyeloid, blast/promyelocyte
Description: Human Caucasian promyelocytic leukaemia
Viruses: See Properties
Depositor: Dr C M Bunce, Immunology Dept, University of Birmingham Medical School, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Differentiation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C. Do not allow the culture medium to become acidic.
Karyotype: 2n = 48, hyperdiploid
Variant of the promyelocytic cell line HL60 (85011431) isolated after treating cells with alpha-particle radiation and then cloned in medium containing 1.25% DMSO. Treatment with 50nM TPA results in only very limited differentiation towards basophilic morphology (Giemsa stain). Differentiation towards monocyte lineage has not been observed.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Exp Hematol 1983;11:828 - PMID: 6641827
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293