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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: human
TISSUE/ORGAN: kidney
TUMOR: adenocarcinoma
ECACC catalog code: 93020513
Morphology: Epithelial
Description: Human kidney adenocarcinoma
Viruses: See Properties
Depositor: Dr S Clark, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x100,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: 45;
Karyotype: 2n = 46, aneuploid
Derived from 78-year-old male. Does not produce tumours in nude mice.
Research council deposit: No;
Price Code: B;
Validated by: DNA fingerprinting * isoenzymes ((species confirmation))
Availability in cell line catalogues: ATCC: HTB 45
Bibliographic references:
- J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
- Oncogene 1994;9(3):939-942 - PMID: 8108138
- Proc Natl Acad Sci U S A 1987;84(22):7972-7976 - PMID: 2825172
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it