A-498

Version 4.0.200811

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A-498 (human, kidney, carcinoma)

SPECIES: human
TISSUE/ORGAN: kidney
TUMOR: carcinoma

DSMZ catalog code: ACC 55
Morphology: epithelial-like, adherent cells growing as monolayers
Description: human kidney carcinoma established from the kidney carcinoma of a 52-year-old man in 1973 confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. Kirchner, Medical School of Hannover, Hannover, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% MEM (with Earle's salts) + 10% FBS split confluent cultures 1:3 to 1:5 every 3-5 days using trypsin/EDTA; seed out at ca. 1.0 x 106 cells/80 cm 2 at 37C with 5% CO2 about 2-3 x 106 cells/80 cm 2; doubling time of > 60 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule; contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypertriploid with 7% polyploidy; 72(68-72)<3n>XX, -X, +2, -3, -6, +7, +7, +9, -11, -14, +17, -18, -20, +21, +3m, dm/r (31%); der(1)t(1;1)(p32;q12), der(2)t(2;3)(q37;q13), i(8q); sideline with der(3)t(3;3)(q27;p14)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofil- vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (negative)

Availability in cell line catalogues: ATCC: HTB 44, IZSBS: BS TCL 111, ICLC: HTL04005

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758