A-427

Version 4.0.200811

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A-427 (human, Caucasian, lung, carcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: lung
TUMOR: carcinoma

DSMZ catalog code: ACC 234
Morphology: epithelial-like adherent cells growing in monolayers
Description: human lung carcinoma established from the lung carcinoma of a 52-year-old Caucasian man confirmed as human with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from DKFZ Tumorbank, Heidelberg, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:5 every 4-5 days using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 25 x 106 cells/80 cm 2; doubling time of about 30-40 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5-3.0 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human near triploid karyotype with 2% polyploidy; 67(59- 72)<3n>XXY, +3, +7, -13, -14, -15, -18, -19, -20, -22, +4mar, add(1)(p11), del(1)(q11), del(3)(p11)x2, der(3)t(1;3)(q21;q11), add(4)(q31), del(6)(q21), del(7)(p11), i(10q), dd(11) * cytogenetics 2 (add(11)(q24), add(14)(p11), add(15)(p13), add(18)(q23); 3p- deletion is characteristic of lung carcinoma) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of AST, NP) * reverse transcriptase (reverse transcriptase not detected)

Availability in cell line catalogues: ATCC: HTB 53, IZSBS: TCL 120

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
Nature 1982;300:539-542 - PMID: 7144906