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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: ATL96006
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: rat, Wistar-Furth (female, 7 months old)
TUMOR: pituitary adenoma
Original line: MtT/W5
DEPOSITOR: Dr T. Florio, Neuroscience Dept, ABC, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The cells were used for studies on growth hormon and prolactin secretion.
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
Ham's F10 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2 37C, 5% CO2., mycoplasma negative, HOECHST and PCR
After thawing the viability of the cells may be low
Validated by: isoenzymes (confirmed as rat with AST, G6PD, PepB)
Bibliographic references:
- J Gen Physiol 1984;83:309-339 - PMID: 6325585
- Proc Natl Acad Sci USA 1985;82:4282-4286 - PMID: 2408278
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it