A 172

Version 4.0.200811

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A 172 (human, nervous system, glioblastoma)

SPECIES: human
TISSUE/ORGAN: nervous system
TUMOR: glioblastoma

ECACC catalog code: 88062428
Description: Human glioblastoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C (medium renewal every 2-3 days recommended).
Passages: 314; Karyotype: n = 80, over 20 marker chromosomes per cell (hypertriploid)
Derived from a glioblastoma removed from a 53 year old male. Non-tumourigenic in anti-thymocyte serum treated NIH Swiss mice.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1620, IZSBS: BS TCL 2

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758