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This is the complete description of the cell line that you requested.
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ICLC catalog code: HTL97015
Continuous culture, grown as monolayer
polygonal flat to spindle-like morphology
SPECIES: human (male, 42 years old) Karyology: complex chromosomal changes; p53 mutation
TISSUE/ORGAN: thyroid
TUMOR: carcinoma, follicular
Derived from: a lymph node metastasis
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The cell line has been used for studies on the function of human thyroid and development of thyroid cancer; expression of 5-deiodinase type I; the cells retained differentiated thyrocyte function and thyrocyte responsiveness to thyrotropin and local active growth factors; thyroglobulin immunoreactivity could be shown in the cytoplasm and EGF receptor immunoreactivity could be visualised on the cell membranes
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + Ham's F12 (1:1) + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-5x104 cells/cm2. 37C, 5% CO2. Passage: 12, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with G6PD, NP.)
Availability in cell line catalogues: ECACC: 94060901
Bibliographic references:
- Front Horm Res 1989;18:56
See also
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it