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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: mouse, C3H
TISSUE/ORGAN: breast
TUMOR: carcinoma
ECACC catalog code: 87111904
Morphology: Lymphoblast
Description: Mouse C3H mammary carcinoma
Viruses: See Properties
Depositor: Dr T Ohno, Riken Gene Bank, Tsukuba, JAPAN
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies, chromosome condensation studies.
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 1-3x100,000 cells/ml; 5% CO2; 33C.
A temperature sensitive mutant isolated from the mouse mammary cell carcinoma cell line, FM3A (ECACC No. 87100804). Cells will Not double at 39C (Non-permissive temperature) and will grow at 33C (permissive temperature). Electron Microscope findings have shown that chromatin abnormally condenses into fragmented and compact forms in 80% of the cells at 39C for 16 hours. This abnormal condensation does Not occur at 33C or in the FM3A (ECACC No. 87100804) cell line at either temperature.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Bibliographic references:
- Exp Cell Res 1980;126:407-416 - PMID: 6153988
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it