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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
Primary culture, grown as monolayer
fibroblast morphology
SPECIES: human, Caucasian (female, 3 years old)
TISSUE/ORGAN: skin, fibroblast
PATHOLOGY: hypercholesterolemia, familial*143890 (OMIM record)
PROPERTIES: cell biology; metabolism; receptor
Available in the following LABORATORY:
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Istituto Patologia Generale, Universita' degli Studi (DSB, Modena)
DMEM + 10% FBS + Antibiotics + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; 37C, 5% CO2, split 1-3 Passage: 5-6
LDL receptor activity compatible with FH heterozygous. Known mutational defect: deletion of exons 14 and 15 and partially of exon 13 of LDL receptor gene.
Validated by: DNA dosage
Bibliographic references:
- Proc Natl Acad Sci U S A 1986;83:3679-3683 - PMID: 3012527
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it