|
Version 4.0.200811 |
|
This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: human
TISSUE/ORGAN: skin, fibroblast
ECACC catalog code: 90011808
Morphology: Fibroblast
Description: Human skin fibroblast
Viruses: See Properties
Depositor: Dr C Arlett, MRC Cell Mutation Unit, University of Sussex, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: DNA repair studies
Available in the following LABORATORY:
-
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 5;
Established from a biopsy of a normal 56 year old male. The cells have been used in radiation survival studies.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Bibliographic references:
- Int J Radiat Biol 1991;59:749 - PMID: 1672363
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it