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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: mouse, (C57BL/6xDBA/2)F1
TISSUE/ORGAN: blood
TUMOR: leukemia, pre-B cell
ECACC catalog code: 91092002
Morphology: Lymphoblastoid
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Description: Mouse pre-B lymphocyte
Viruses: See Properties
Depositor: Dr P Kincade, Oklahoma Medical Research Foundation, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Cytoplasmic Immunoglobulin
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS). Maintain cultures between 2-5x100,000 cells/ml; 5% CO2; 37C.
Derived from a methyl nitrosourea-induced tumour in a (C57BL/6XDBA/2) F1 mouse. This line is positive for cytoplasmic Ig and negative for surface membrane Ig and TdT.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: TIB 158
Bibliographic references:
- J Immunol 1978;121:641 - PMID: 308074
- Leuk Res 1979;3:23 - PMID: 316844
- Nature 1981;292:631 - PMID: 6789213
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it