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ECACC catalog code: 89020802
Susceptibility to viruses: rabies
Description: Rabbit embryo skin
Viruses: See Properties
Depositor: Prof F Horaud, Institut Pasteur, Paris, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: rabies virus
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from trypsinised skin. The cells entered a 'crisis' period after 3 passages and subsequently stabilised. Remain stable for at least 100 passages. Susceptible to rabies virus, producing similar titres as those from Vero cells.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Dev Biol Stand 1985;60:133 - PMID: 4043531
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293