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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HYL96001
Continuous culture, grown as suspension
single or clustered, round cells morphology
SPECIES: mouse, C57BL/6
TUMOR: hybridoma, B cell
DEPOSITOR: Dr T. Florio, Neuroscience Dept, ABC, Genoa, Italy.
Cell line available for distribution,only as frozen ampoule, upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: IL-6 dependent hybridoma obtained by fusion of spleen cells from a mouse immunized with 50 ug Escherichia coli lipopolysaccharide, indicator cell line for IL-6.
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
Iscove's modified Dulbecco's medium + 10% FBS + 2mM L-Glutamine + 0.24mM L-asparagine + 0.55mM L-arginine + 0.1mM Hypoxantine + 50uM 2-mercaptoethanol + 25ng/ml IL-6; Freezing medium: Culture medium + 50% FBS + 10% DMSO; maintain cultures between 3 and 9 x 105 cells/ml; split 1:5-1:10. 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Initially after thawing the viability of the cells may be low
Validated by: isoenzymes (confirmed as mouse with MD, NP)
Bibliographic references:
- Proc Natl Acad Sci USA 1986;83:9679-9683 - PMID: 2948184
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it