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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: human
TISSUE/ORGAN: B cell
PATHOLOGY: chronic Hepatitis C (OMIM
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ECACC catalog code: 94111519
Morphology: Lymphoblast
Description: Human IgG1 secreting B cell
Viruses: See Properties
Depositor: Prof M Mondelli, Dr A Cerino, Instituto di Clinica delle Malatte Infettive, IRCCS Policlinico San Matteo, Pavia, ITALY (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Immunoglobulin G (IgG1) (kappa) specific for Hepatitis C virus NS4 region Applications: Used in HCV typing studies.
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 20u/ml IL-6 + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x10,000 cells/ml; using 0.25% trypsin or trypsin EDTA; 5% CO2: 37C.
Passages: +3;
Derived from a patient with chronic Hepatitis C (HCV type 1). Antibody specific to non structural protein 4a (NS4a) of Hepatitis C virus. Fine specificity mapped to amino acid residues 1697-1708 of HCV deduced amino acid sequence. The cells should be handled under laboratory containment level 2 conditions.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Bibliographic references:
- J Immunol 1991;147:2692 - PMID: 1717573
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it