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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: AL97004
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: horse (female, 4 years old)
TISSUE/ORGAN: dermis
Susceptibility to viruses: coronavirus * equine infectious anaemia virus * herpes simplex * reovirus 3 * vesicular stomatitis (Ogden strain)
DEPOSITOR: Dr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
MEM (EBSS) + 10% FBS + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:2-1:3 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2. Finite lifespan of approximately 40 passages. Passage: 20, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as horse with NP)
Availability in cell line catalogues: IZSBS: BS CL 40, ECACC: 88032803
Bibliographic references:
- Am J Vet Res 1981;42:1501-1505 - PMID: 6275755
- Am J Vet Res 1986;47:1931-1934 - PMID: 3021027
- Int J Cancer 1975;15:171-179 - PMID: 165152
See also
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it