DU-145

Version 4.0.200811

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DU-145 (human, prostate, carcinoma)

SPECIES: human
TISSUE/ORGAN: prostate
TUMOR: carcinoma

DSMZ catalog code: ACC 261
Morphology: epithelial-like adherent cells growing as monolayers
Description: human prostate carcinoma established from the tumor tissue removed from the metastatic central nervous system lesion of a 69-year-old man with prostate carcinoma in 1975 confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from DKFZ Tumorbank, Heidelberg, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 35 x 106 cells/175 cm 2; doubling time of about 30-40 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotriploid karyotype with 12% polyploidy; 62(58- 65)<3n>X, -X/Y, -X/Y, -2, -3, +5, -8, -10, -13, +15, +15, -16, -18, -19, - 20, -21, -22, +3mar, del(1)(p31), del(2)(p11), i(5p), del(6)(q22), del(9)(p12), del(11)(q23), der(12)t( 1;12) * cytogenetics 2 ((q11;p11), add(13)(q33), add(13)(q33), add(15)(p11)x2, add(16)(q24); closely resembles reported karyotype) * DNA fingerprinting (unique DNA pofile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+, desmin-, endothel-, GFAP-, neurofilament-, vimentin+) * reverse transcriptase (reverse transcriptase not detected)

Availability in cell line catalogues: ATCC: HTB 81, ICLC: HTL99013

Bibliographic references:

Cancer Res 1977;37:4049 - PMID: 908039
Int J Cancer 1978;21:274 - PMID: 631930
Models for Prostate Cancer (G P Murphy ed) 1980;67-84