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SPECIES: human, Caucasian
TUMOR: carcinoma, small cell
ECACC catalog code: 95062823
Description: Human Caucasian lung small cell carcinoma
Viruses: See Properties
Depositor: Prof OS Pettengill and Dr G D Sorenson, Dartmouth Medical School, Lebanon, NH, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Bombesin (BN), Epidermal growth factor (EGF), Transforming growth factor (TGF-beta), acetylcholine receptor (AChR) Products: Variety of hormones, high level of calcitonin Applications: Human lung tumour model
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Waymouth's MB 752/1 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS) (Heat Inactivated). Split confluent cultures 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No 60
DMS 53 is a small cell carcinoma of the lung (SCLC) isolated in 1974 from a mediastinal biopsy at time of diagnosis from a 54 year-old male patient prior to treatment. DMS 53 secrete a large variety of hormones including adrenocorticotropin (ACTH), bombesin (BN), calcitonin (CT), chorionic gonadotrophin (hCG), glucagon, growth hormone (GH), oxytocin-neurophysin (OT-NP), parathyroid hormone (PTH), somatostatin-like immunoreactivity (SRIF), thyroid releasing hormone (TRH) and 17beta-estradiol. Expression of cell surface antigens Leu-7, Class I HLA, CLASS II HLA and My23 (defined by AML-2-23 monoclonal antibody) has been shown. DMS 53 cells produce receptors for BN, epidermal growth factor (EGF), transforming growth factor-beta (TGF beta) and acetylcholine (AChR). This cell line was shown to be tumourigenic in nude, athymic mice.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 2062
- Cancer 1980;45:906 - PMID: 7397666
- Cancer 1981;47:1289 - PMID: 6261922
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293