5637

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

5637 (guinea pig, 2/N, fetal)

SPECIES: guinea pig, 2/N
TISSUE/ORGAN: fetal

ECACC catalog code: 90110524
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Description: Guinea pig fetal cells transformed
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-5 x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Passages: 32; Karyotype: Diploid
Derived from a female foetal carcass of a 2/N guinea pig. The cells were treated with benzopyrene for 7 days and a clone selected at the 20th passage. The cells are tumourigenic in guinea pigs.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1405, DSMZ: ACC 35, ICLC: HTL01017

Bibliographic references:

Cancer Res 1975;35:1035 - PMID: 1116142
Cancer Res 1975;35:1045 - PMID: 1116143
Cancer Res 1976;36:128 - PMID: 1247991