|
Version 4.0.200811 |
|
This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
Original line: CV-1
TRANSFORMED BY: 25
PROPERTIES: transfection; virus replication
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1650, IZSBS: BS CL95, ECACC: 88031701, DSMZ: ACC 63, ICLC: ATL01002
Bibliographic references:
- Cell 1981;23:175-182 - PMID: 6260373
DSMZ catalog code: ACC 63
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
TRANSFORMED BY: 25
Available in the following LABORATORY:
-
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
mycoplasma negative, DAPI, broth-agar, hybridization
Validated by: cytogenetics 1 (hypotetraploid simian karyotype with 4% polyploidy; 102(95-108)<4n>; structural rearrangements present) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile (identical to COS-7)) * isoenzymes (confirmed as of primate origin with IEF of NP, PEP B)
Availability in cell line catalogues: ATCC: CRL 1650, IZSBS: BS CL95, ECACC: 88031701, ICLC: ATL01002
Bibliographic references:
- Cell 1981;23:175-182 - PMID: 6260373
ECACC catalog code: 88031701
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
TRANSFORMED BY: 25
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1650, IZSBS: BS CL95, DSMZ: ACC 63, ICLC: ATL01002
Bibliographic references:
- Cell 1981;23:175-182 - PMID: 6260373
ICLC catalog code: ATL01002
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
Original line: CV-1
TRANSFORMED BY: 25
DEPOSITOR: Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: This line contains T antigen. Suitable for transfection by vectors requiring expression of SV40 T antigen
Available in the following LABORATORY:
-
Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:4-1:10 using trypsin/EDTA; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: PCR analysis (confirmed as African green monkey with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: IZSBS: BS CL95, ECACC: 88031701, DSMZ: ACC 63
Bibliographic references:
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-295
Email: assunta.manniello@istge.it