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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL96015
Continuous culture, grown as monolayer
neuroblast-like and epithelial-like, at confluence forms loosely adherent aggregates morphology
SPECIES: human, Caucasian (male, 22 months old)
TISSUE/ORGAN: bone marrow
TUMOR: neuroblastoma
DEPOSITOR: Obtained from ECACC
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells are multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
MEM (EBSS) + Ham's F12 (1:1) + 10% FBS + 2 mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2. Passage: 29 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with G6PD, MD, NP)
Availability in cell line catalogues: ECACC: 95011815
Bibliographic references:
- Cancer Res 1978;38:3751-3757 - PMID: 29704
- J Natl Cancer Inst 1976;57:683-695 - PMID: 62055
- Life Sci 1991;50:7-11
- Science 1976;191:185-187 - PMID: 942798
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-295
Email: assunta.manniello@istge.it