<br>SK-HEP-1<br>

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

SK-HEP-1 (human, Caucasian, liver, adenocarcinoma)

DSMZ catalog code: ACC 141
SPECIES: human, Caucasian
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
mycoplasma negative, DAPI, broth-agar, hybridization
Validated by: cytogenetics 1 (hypotriploid karyotype with 9% polyploidy; 62/63(54-64)<3n>X/XX, -Y, -3, -4, +6, +7, -9, -13, -13, -13, -14, -15, -16, -16, +17, -18, +19, +20, -21, +22, +mar, der(1)t(1;?)(q21;?), t(2;20)(p11;p11), del(6)(q25), der(14)t(14;?) * cytogenetics 2 (i(14q), der(19)t(19;?)(p13;?); sidelines carry t(14q15q) for i(14q) and der(12)t(12;?)(p12.3;?)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin++, desmin-, endothel-, GFAP-, neurofilament-, vimentin++) * isoenzymes (confirmed as human with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: HTB 52, ECACC: 91091816

Bibliographic references:

Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047

SK-HEP-1 (human, Caucasian, liver, adenocarcinoma)

ECACC catalog code: 91091816
SPECIES: human, Caucasian
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
mycoplasma negative, HOECHST and culture
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: HTB 52, DSMZ: ACC 141

Bibliographic references:

Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047

SK-HEP-1 (human, Caucasian, liver, adenocarcinoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 52 years old) Karyology: hyperdiploid to hypotriploid
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: bFGF prod.; tumorigenicity

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)
mycoplasma negative
Tissue: liver, ascites fluid.

Availability in cell line catalogues: ATCC: HTB 52, ECACC: 91091816, DSMZ: ACC 141

Bibliographic references:

Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047

For information, get in touch with:

Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy Tel: +39-0105558-289, Fax: +39-0105558-295
Email: assunta.manniello@istge.it