<br>NRK-49F<br>

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

NRK-49F (rat, kidney)

Monoclonal continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: rat
TISSUE/ORGAN: kidney
PROPERTIES: contact inhibition; transformation; chemical transformation; viral transformation

Available in the following LABORATORY:

Consorzio Mario Negri SUD, Centro di Ricerche Biomediche e Farmacologiche (CHIBC, S.Maria Imbaro (CH))
DMEM + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-3 Passage: 31 , mycoplasma negative
Validated by: semisolid agar colonies

Availability in cell line catalogues: ATCC: CRL 1570, ECACC: 86101301, DSMZ: ACC 172

Bibliographic references:

J Bacteriol 1966;92:1133-1140 - PMID: 5926742
J Cell Physiol 1978;94:335 - PMID: 304450

NRK-49F (rat, kidney, fibroblast)

DSMZ catalog code: ACC 172
SPECIES: rat
TISSUE/ORGAN: kidney, fibroblast

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
mycoplasma negative, DAPI, broth-agar, hybridization
Validated by: cytogenetics 1 (near-diploid rat karyotype with 4% polyploidy; 40(37-42)<2n>) * isoenzymes (confirmed as rat with IEF of AST, NP, PEP B)

Availability in cell line catalogues: ATCC: CRL 1570, ECACC: 86101301

Bibliographic references:

J Bacteriol 1966;92:1133-1140 - PMID: 5926742
J Cell Physiol 1978;94:335 - PMID: 304450

NRK-49F (rat, kidney)

ECACC catalog code: 86101301
SPECIES: rat
TISSUE/ORGAN: kidney

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
mycoplasma negative, HOECHST and culture
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1570, DSMZ: ACC 172

Bibliographic references:

J Bacteriol 1966;92:1133-1140 - PMID: 5926742
J Cell Physiol 1978;94:335 - PMID: 304450

NRK-49F (rat, kidney)

Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: rat
TISSUE/ORGAN: kidney
PROPERTIES: contact inhibition; transformation; chemical transformation; viral transformation

Available in the following LABORATORY:

Istituto Nazionale per lo Studio e la Cura dei Tumori (MIISB, Milano)
DMEM + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-3

Availability in cell line catalogues: ATCC: CRL 1570, ECACC: 86101301, DSMZ: ACC 172

Bibliographic references:

J Bacteriol 1966;92:1133-1140 - PMID: 5926742
J Cell Physiol 1978;94:335 - PMID: 304450

For information, get in touch with:

Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy Tel: +39-0105558-289, Fax: +39-0105558-295
Email: assunta.manniello@istge.it