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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old) Karyology: hypertriploid to hypotetraploid
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
Endogenous viruses: contains type C * contains type B
PROPERTIES: estrogen receptor positive; specialized product secr.; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity; virology; cell biology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 22, ECACC: 86012803, ICLC: HTL95021
Bibliographic references:
- J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
ICLC catalog code: HTL95021
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old) Karyology: hypertriploid to hypotetraploid
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
Derived from: pleural effusion
tumorigenic in nude mice
Endogenous viruses: contains type C * contains type B
DEPOSITOR: Dr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: estrogen receptor positive; specialized product secretion; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
The viability may be low after thawing; thaw with medium supplemented with 20% FBS
Validated by: isoenzymes (confirmed as human with G6PD, NP)
Availability in cell line catalogues: ECACC: 86012803
Bibliographic references:
- Cancer Res 1990;118:48 - PMID: 2152773
- J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old) Karyology: hypertriploid to hypotetraploid
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
Endogenous viruses: contains type C * contains type B
PROPERTIES: estrogen receptor positive; specialized product secr.; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity; virology; cell biology
Available in the following LABORATORY:
-
Boehringer Mannheim Italia S.p.A. (MIEOS, Monza (MI))
MEM + 10% FBS + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, split 1-5 Passage: 143 , mycoplasma negative
Validated by: isoenzymes (PGM3,1; PGM1,1-2; ES D,1-2; AK1,1; GLO-1,1-2; G6PD,B)
Availability in cell line catalogues: ATCC: HTB 22, ECACC: 86012803, ICLC: HTL95021
Bibliographic references:
- J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old) Karyology: hypertriploid to hypotetraploid
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
Endogenous viruses: contains type C * contains type B
PROPERTIES: estrogen receptor positive; specialized product secr.; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity; virology; cell biology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 22, ECACC: 86012803, ICLC: HTL95021
Bibliographic references:
- J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old) Karyology: hypertriploid to hypotetraploid
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
Endogenous viruses: contains type C * contains type B
PROPERTIES: estrogen receptor positive; specialized product secr.; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity; virology; cell biology
Available in the following LABORATORY:
-
Russian Academy of Sciences (SPBIC, St. Petersburg)
mycoplasma negative
The ability to process estradiol via cytoplastic estrogen receptors and to form domes.
Availability in cell line catalogues: ATCC: HTB 22, ECACC: 86012803, ICLC: HTL95021
Bibliographic references:
- J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 69 years old) Karyology: hypertriploid to hypotetraploid
TISSUE/ORGAN: breast
TUMOR: adenocarcinoma
tumorigenic in nude mice
Endogenous viruses: contains type C * contains type B
PROPERTIES: estrogen receptor positive; specialized product secr.; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity; virology; cell biology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 22, ECACC: 86012803, ICLC: HTL95021
Bibliographic references:
- J Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-295
Email: assunta.manniello@istge.it