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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL08002
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, 43 years old)
TISSUE/ORGAN: skin
TUMOR: melanoma, metastatic
Derived from: lymph node metastasis
DEPOSITOR: Dr F. Tosetti, Cellular Biology Dept, IST at ABC, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM-Glutamine + 4,5 g/L glucose; Freezing medium: Culture medium 45% + 50% FCS + 5% DMSO; split confluent cultures1:3-1:6; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: ECACC: 91100402
Bibliographic references:
- Proc Natl Acad Sci U S A. 1977;74(2):565-9 - PMID: 265522
- Proc Natl Acad Sci U S A. 1979; 76(3):1288-92 - PMID: 375235
- Proc Natl Acad Sci U S A. 1980;77(9):5258-62 - PMID: 6254071
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-295
Email: assunta.manniello@istge.it